REF-011 · Roberts et al., 2015 · The Journal of Physiology, Vol. · Paper

Cold water immersion after strength training blunts muscle and strength gains

B+moderate evidenceMeasured on trained athletesWritten before October 2026, being re-checked

Participants

n=21 physically active men (12-week study); a further 9 active men in the acute biopsy study

Protocol

Study 1: 12 weeks of strength training, 2 days per week, each session followed by 10 min of cold water immersion (CWI) or active recovery (ACT)
Study 2: single-leg strength exercise followed by CWI or ACT on separate days; biopsies before and 2, 24 and 48 h after

Key Results

Metric (12 weeks)Active RecoveryCWIp
Strength and muscle massincreased moresmaller gain<0.05
Isokinetic work+19%no significant increase<0.05
Type II fibre CSA+17%no significant increase<0.05
Myonuclei per fibre+26%no significant increase<0.05

Acute study: satellite cells rose 24-48 h after exercise with ACT (NCAM+ 10-30%, Pax7+ 20-50%); with CWI only NCAM+ rose, and only at 48 h. Both satellite-cell counts were greater after ACT than CWI (p<0.05). p70S6 kinase phosphorylation rose in both conditions but more after ACT (p<0.05).

Mechanism

CWI attenuates the acute satellite-cell response and the activity of kinases that regulate hypertrophy (p70S6K, downstream of mTOR); the authors suggest this translates into the smaller long-term gains. It is an attenuation, not a complete block — strength and mass still increased in the CWI group, just less.

Practical Application

Finding: in 21 active men, 10 min of cold water immersion after every strength session for 12 weeks cut the gains: active recovery gave +19% isokinetic work, +17% type II fibre area and +26% myonuclei per fibre, none of which rose significantly with CWI; the acute satellite-cell and p70S6K responses were blunted for up to 2 days. Do not ice-bath after the gym if the gym is meant to build muscle or strength.
Not from this paper; the base's own practice: keep CWI for recovery after pure endurance sessions or in competition, away from strength work.
CWI and sleep (not from this paper — REF-019 Chauvineau 2021): whole-body CWI raised slow-wave-sleep proportion in the first 180 min of the night vs partial immersion (n=12 runners; no effect size reported; provisional).

Limitations

Physically active men, not trained athletes; strength training only 2 days per week; a 10 min CWI protocol — other temperatures/durations were not tested
"Attenuated" gains: the abstract does not give the CWI group's own percentage changes
How this entry was checked and changed
Why this level

regraded 2026-09-27 from A: parallel controlled trial, n=21, plus acute n=9 mechanistic arm

Content correction 2026-09-27
Table "CWI 0%" -> abstract says the CWI group did not increase significantly and that strength and mass "increased more" in ACT; wording corrected. "Satellite cells +10-50%" -> NCAM+ 10-30% and Pax7+ 20-50% with ACT.
Mechanism "complete blockade of hypertrophy and strength adaptations" -> attenuation (p70S6K phosphorylation and satellite cells lower after CWI, not absent).
Sport "cycling, running, strength" -> strength (the study was strength training only). Level A -> B+ (parallel trial n=21). Second acute study (n=9) added; "NEVER" rule relabelled as the base's own practice.
Full text (checked 2026-10-12)
Study 1 was a randomized, controlled trial: 24 physically active young men with at least 12 months of experience in strength training were matched in pairs and allocated to CWI (n = 12) or active recovery (n = 12); three withdrew because of injury not associated with the study (full text).
Training was mainly lower-body, twice a week separated by 72 h, at 8-, 10- and 12-repetition maximum loads, with leg press, knee extension, knee flexion, walking lunges and plyometric exercises (full text).
CWI was started within 5 min after each session: 10 min seated with both legs immersed up to the waist in water at 10.1 ± 0.3 °C; active recovery was 10 min of self-selected low-intensity cycling at a mean 59.5 ± 9.4 W (full text).
Muscle mass increased by 309 ± 73 g with active recovery and 103 ± 71 g with CWI; the between-group difference was 206 g (d = 4.1; P < 0.001) (full text).
1-RM increased in both groups: leg press 201 ± 65 kg (active recovery) vs 133 ± 43 kg (CWI) and knee extension 33.8 ± 8.5 kg vs 17.8 ± 9.2 kg, with between-group differences of 57 kg (d = 1.5) and 15.6 kg (d = 1.4) (full text).
Maximal isometric torque rose by 65 ± 32 Nm with active recovery but not with CWI (P = 0.11); RFD impulse rose in both groups (164 ± 130 vs 51 ± 44 Nm·s-1) (full text).
Study 2 was a randomized cross-over study in 10 physically active young men (one withdrew), with CWI water at 10.3 ± 0.5 °C and active recovery cycling at 36.6 ± 13.8 W (full text).
p70S6K phosphorylation was greater after active recovery than CWI at 2 h (90%; d = 4.7) and 24 h (60%; d = 5.3) after exercise (full text).