REF-134 · Matomäki et al., 2025 · International Journal of Sports Physiology and Performance, Vol. · Paper

Substituting Low-Intensity Endurance Exercise With High-Intensity Microintervals: Responses to Acute Exercise

B+moderate evidenceMeasured on trained to highly trained athletesWritten before October 2026, being re-checked

Participants

n=13 endurance athletes (abstract); cycling exercise. Regional- to international-level athletes (5 triathletes, 4 cyclists, 2 dog skijorers, 1 runner, 1 rugby player; 6 women, 7 men), all spending at least 40% of their training time cycling; approved by the University of Jyväskylä ethics committee, Finland (accepted-manuscript full text, checked 2026-09-28: Methods, Table 1).

Protocol

4 sessions of 45 minutes in randomized crossover:

ConditionDescription
CONContinuous low-intensity work at 88% LT1 power (classic Z1)
MI 4-26Microintervals, work:rest 4 s:26 s (a work interval starts every 30 s): alternating 111% of the power associated with VO2max and ~60 W (accepted-manuscript full text, checked 2026-09-28: Methods)
MI 7-23Microintervals, work:rest 7 s:23 s: same intensities
MI 10-20Microintervals, work:rest 10 s:20 s: same intensities

Key point: work phase at 111% of power at VO2max — very high muscular intensity, but so brief (the paper defines microintervals as ≤15 s at ~100% VO2max) that the systemic metabolic response stays near LT1.

Key Findings

MeasureCON vs MI 4-26CON vs MI 7-23CON vs MI 10-20
VO2Did not exceed LT1 more than CON (p≥.26, ES≤0.44)SameSame
HRDid not exceed LT1 more than CONSameSame
LactateNo differences (ANOVA p≥.36)No differencesNo differences
RER (mean)No differencesNo differencesNo differences
Fat oxidationDid not exceed CONSameSame
RPENo differenceNo differenceHigher: 3.1±0.8 vs 2.4±0.7 (p=.01)

Equivalence: all mean responses of MI 4-26 and MI 7-23 were statistically equivalent to CON (equivalence test p≤.02).

Variable oscillation:

HR: oscillation >100% greater with microintervals vs CON (p≤.001, ES≥1.7)
VO2: oscillation at most 25% greater (p≥.02, ES≤0.70)

Mechanism

Inference, not tested in this paper: high-intensity muscular work in ultra-short work phases does not raise systemic VO2 or lactate above LT1 because phosphocreatine and oxygen stores cover the work-phase demand and the ~60 W recovery phases allow rapid restoration; fast-twitch fibres are recruited per sprint, yet the whole-body metabolic response remains aerobic. The authors describe the class as "high-intensity muscular work blended with mild metabolic perturbation".

Main Conclusion

Microintervals = "oscillating low-intensity exercise" (authors' term). They fulfil the traditional low-intensity criteria: responses of almost all internal variables — including fat oxidation — did not exceed those of continuous work at 88% LT1. Classifying them as HIT by systemic response would be incorrect, despite the high muscular power.

Practical Application

In n=13 endurance athletes, 45 min of microintervals alternating 111% of power at VO2max with ~60 W at work:rest 4 s:26 s or 7 s:23 s produced HR, VO2, lactate, RER, fat oxidation and RPE equivalent to 45 min of continuous riding at 88% LT1 (equivalence p≤.02); only 10 s:20 s raised RPE (3.1 vs 2.4, p=.01). HR oscillated >100% more, VO2 at most 25% more. Very short sprints inside an easy ride can stay Z1 by every systemic marker.
Not from this paper; the base's own practice: replace part of a Z1 ride with 4 s:26 s or 7 s:23 s microintervals when a neuromuscular stimulus is wanted without adding metabolic load — for example in a deload week or inside a long base ride. The paper tested 45-min sessions only; substitution amounts and weekly placement are house decisions.
The 10 s:20 s configuration costs more perceived effort at the same systemic response — prefer 4 s:26 s or 7 s:23 s on days that must feel easy.
Inference, not tested: because the systemic response stays at Z1, microintervals should not shift the polarized 80/20 distribution (REF-087, REF-303) — but their chronic training effect is unknown (acute study).

Limitations

Only acute responses — unknown whether chronic microinterval training produces adaptations beyond standard Z1. Single work-phase intensity (111% of power at VO2max). Cycling only, 45-min sessions only. n=13. Randomized longitudinal research needed for chronic effects.

How this entry was checked and changed
Content correction 2026-09-27
Sport "cycling, strength, sprint" -> cycling (the study is a cycling protocol; strength and sprint are not disciplines studied).
"Replace 20-30 min pure Z1", "2×15min microintervals", "Type II recruitment without metabolic stress" -> not in the abstract; substitution amounts relabelled as the base's own practice, fibre-recruitment mechanism labelled inference.
"Fat oxidation preserved" -> abstract wording: responses, fat oxidation included, did not exceed CON.
Cross-references to duplicate stubs repointed to their canonical entries (REF-085->REF-303); the stubs were emptied on 2026-09-27.
2026-09-28: conditions "4-6 s / 7-23 s / 10-20 s cycles" -> work:rest 4 s:26 s, 7 s:23 s and 10 s:20 s, a work interval every 30 s (accepted manuscript, Methods). The published abstract's Methods prints "4-6" while its Results say "4-26"; "4-6" is a typo.
2026-09-28: lines labelled "full text; not checked" read against the open-access accepted manuscript (JYX, University of Jyväskylä repository, jyx.jyu.fi): 1 confirmed (Jyväskylä; participant composition added), 0 corrected, 0 removed.
2026-09-28: microinterval conditions corrected against the open-access accepted manuscript (jyx.jyu.fi, Methods): "4-6 / 7-23 / 10-20 s cycles" -> work:rest 4 s:26 s, 7 s:23 s, 10 s:20 s in the protocol table, findings table, equivalence line and practical application; the published abstract's "4-6" is a typo for 4-26.